Open AccessNatural Resistance Associated Macrophage Protein-1 Expression in Salmonella typhi Infection with Acute Recurrence State of Typhoid Fever in Endemic Areas, Indonesia
Author(s) -
Ade Rifka Junita,
Firdaus Hamid,
Rosdiatzir,
Rosana Agus,
Burhanuddin Bahar,
Mochammad Hatta
Publication year - 2020
Publication title -
microbiology research journal international
Language(s) - English
Resource type - Journals
ISSN - 2456-7043
DOI - 10.9734/mrji/2020/v30i930258
Subject(s) - typhoid fever , salmonella typhi , medicine , salmonella , immunology , pathogenesis , virology , bacteria , biology , gene , biochemistry , genetics , escherichia coli
Background and Aim: The molecular pathogenesis of typhoid fever is complex and the host susceptibility mechanisms such as Natural Resistance Associated Macrophages Protein-1 (NRAMP-1) are poorly understood. This study explores the expression of NRAMP-1 in the serum of Acute Recurrence State (ARS) of typhoid fever compared with typhoid fever patients and healthy persons.
Methods: Thirty of ARS of typhoid fever and 30 typhoid fever patients were collect from several Primary Health Care and Hospitals in the endemic area. Diagnosis of typhoid fever based on clinical symptoms and confirmed by blood culture. Healthy persons were collect from the Blood Transfusion Unit, Makassar, Indonesia. The expression of NRAMP-1 was determined using Enzyme-linked immunosorbent assay (ELISA).
Results: The mean of NRAMP-1 expression in 30 ARS of typhoid fever and 30 typhoid fever were found 10.941,56 pg/mL and 11.027,65 pg/mL, respectively. However, the mean of NRAMP-1 expression in healthy persons was found 21.103,91 pg/mL.
Conclusion: No different the expression of NRAMP-1 in ARS of typhoid fever compared with typhoid fever patients. However, expression of NRAMP-1 in both ARS of typhoid fever and typhoid fever showed significantly lower than healthy persons. Future study is needed to explore the other molecular factors involved to become ARS.