Open AccessAntibacterial Screening and Analysis of Streptomyces coelicolor Secondary Metabolites
Author(s) -
Aqeel I. Gheni,
Ayad H. Hasan
Publication year - 2022
Publication title -
journal of pharmaceutical research international
Language(s) - English
Resource type - Journals
ISSN - 2456-9119
DOI - 10.9734/jpri/2022/v34i7b35469
Subject(s) - actinorhodin , streptomyces coelicolor , staphylococcus aureus , streptococcus pyogenes , microbiology and biotechnology , agar diffusion test , agar , antibacterial activity , minimum inhibitory concentration , biology , streptomyces , chemistry , bacteria , antimicrobial , genetics
Aims: The widespread usage of commercially accessible antibacterial agents has resulted in the emergence of multidrug-resistant bacterial pathogens worldwide. Streptomycetes produce a variety of antibacterial secondary metabolites and the study, as a result, will look into the antibacterial potential of Streptomyces coelicolor against some pathogenic bacteria.
Place and Duration of Study: The study was based at the department of Medical Microbiology, Science and Health research center, Faculty of science and health, Koya University. between April, 2019 and December, 2020.
Methodology: The Streptomyces coelicolor strains in this study were verified phenotypically via tryptone soya agar in addition to their genotype using three different sets of primers. Afterwards, Thin Layer Chromatography (TLC) was utilised to purify actinorhodin from the crude extract of S. coelicolor secondary metabolites. The antibacterial activities of the actinorhodin and the crude extract have further been tested against standard strains of Staphylococcus aureus (ATCC 25923), Streptococcus pyogenes (ATCC 19165), Escherichia coli (ATCC 25218), and Salmonella typhi (ATCC 14028). Disc diffusion and minimum inhibitory concentration (MIC) techniques were both used to serve this purpose.
Results: A significant activity of the organic solvent extract was observed against Staphylococcus aureus and Streptococcus pyogenes using the disc diffusion method with diameter zone inhibition of (9-20 mm) and (11-23 mm), respectively. Actinorhodin, on the other hand, showed a reasonable wider effect against them with diameter zone inhibition of (10-24 mm) against S. aureus and (10-27 mm) against S. pyogenes. Comparatively, the MIC test showed akin results by inhibiting S. aureus and S. pyogenes at a MIC value of 16 µg/ml using crude extract. Whereas actinorhodin expressed greater inhibitory activities against S. aureus, S. pyogenes and S. typhi with MICs of (8, 16 and 32 µg/ml), respectively.
Conclusion: The capability of suggested actinorhodin from the L646 strain to inhibit Staphylococcus aureus and Streptococcus pyogenes growth with a MIC value of 8 g/ml was a remarkable finding obtained by studying Streptomyces coelicolor secondary metabolites.