Open AccessSpectrophotometric Assessment of Benzene 1,3,5-Triol in Pure Forms and in Bulk Sample by Diazotiization Coupling Reaction
Author(s) -
Mohauman Mohammad Al-Rufaie,
Aymen Abdul Rasool Jawad,
Lamia Abdultef Risan Al-Iessa
Publication year - 2022
Publication title -
journal of pharmaceutical research international
Language(s) - English
Resource type - Journals
ISSN - 2456-9119
DOI - 10.9734/jpri/2022/v34i6b35433
Subject(s) - phloroglucinol , molar absorptivity , chemistry , aniline , benzene , triol , absorption (acoustics) , phenol , aqueous solution , color reaction , analytical chemistry (journal) , nuclear chemistry , chromatography , materials science , organic chemistry , reagent , diol , optics , physics , composite material
The method was published for determining microgram quantities of benzene 1,3,5-triol (phloroglucinol) in aqueous solution and some bulk samples using an easy, quick, and effective spectrophotometric method. The method uses a diazotization as well as coupling process in basic medium to produce a bright yellow water-soluble dye from Phloroglucinol and diazotized 4-Methoxy aniline That is stable and also has a maximum absorption wavelength of 420 nm. With a molar absorptivity of 1.5989x 104l mol-1 cm-1 as well as index of Sand ell sensitivity 0.001 g.cm- 2, Beer's law is followed across a concentration range of (2-40) g.ml-1 of phloroglucinol.The ideal conditions for all color development are detailed, and even the suggested procedures for determining phloroglucinol in aqueous medium as well as some bulk sample have been successfully employed.