Open AccessEffect of Carica Seed Extract on Inhibitory Kappa B Kinase Beta and mTOR mRNA Expression in Lung Cancer Cells (A549 Cells)
Author(s) -
Ridha Azimudin,
S. Preetha,
Selvaraj Jayaraman,
G. Sridevi,
M. Jeevitha
Publication year - 2022
Publication title -
journal of pharmaceutical research international
Language(s) - English
Resource type - Journals
ISSN - 2456-9119
DOI - 10.9734/jpri/2022/v34i5b35410
Subject(s) - carica , lung cancer , pi3k/akt/mtor pathway , a549 cell , kinase , cancer cell , cancer , mtt assay , messenger rna , medicine , biology , microbiology and biotechnology , pharmacology , traditional medicine , in vitro , apoptosis , botany , biochemistry , oncology , gene
Background: Lung cancer is considered as one of the most common causes for cancer-related death globally. Carica papaya is one of the most well-known traditional medicines to treat diseases, and is also known to treat cancer and help in cancer prevention. The study investigates the effect of Carica seed extract on inhibitory kappa B kinase beta and mTOR mRNA expression in lung cancer cells (A549 cells).
Methods: Cell viability test was assessed using MTT assay. mRNA expression of inhibitory kappa B kinase beta and mTOR mRNA was analyzed by real-time PCR. The results was analysed statistically by ANOVA and Duncan multiple range test with graphpad prism version 5 software. p<0.05 was considered significant.
Results: It was observed that there wasmaximum inhibition (50%) at 400-500µg/ml of Carica seed extract. It was also found that the fold change over control of mTOR mRNA expression was significant at 500µg/ml of Carica seed extract and the fold change over control of IKKB mRNA expression was significant (p<0.05) at 400µg/ml in cancer cells treated with Carica seed extract.
Conclusion: Thus concluding that Carica seed extract has been found to have significant anticancer property on A549 lung cancer cell lines, and can be used as a natural product in combating lung cancer.