Open AccessThe Significance of Nigella sativa on Histomorphological Variations of Seminiferous Tubules in Doxorubicin Treated Albino Rats
Author(s) -
Ashok Kumar,
Anjum Rehman,
Sadia Sundus,
Saher Fatima,
Zeeshan Ahmed,
Asma Aijaz
Publication year - 2022
Publication title -
journal of pharmaceutical research international
Language(s) - English
Resource type - Journals
ISSN - 2456-9119
DOI - 10.9734/jpri/2022/v34i12a35548
Subject(s) - nigella sativa , doxorubicin , atrophy , medicine , traditional medicine , dose , pharmacology , chemotherapy
Objective: To analyze the impact of nigella sativa on Histomorphological changes in doxorubicin treated albino rats.
Research Design: Experimental research.
Abode of Study: Anatomy Department, BMSI, Karachi, Pakistan.
Materials and Methods: Piloted on 40 albino rats for 35 days in the Anatomy Department of BMSI. They were 3-4 months old and about 221 to 238 gm in weight. Rats were separated into 4 groups, Ao, Bo, Co & Do. Ao represented as control, Bo recieved Doxorubicin (DOX) 0.003g/1000g/ at 7TH day intraperitonealy, Co recieved Nigella sativa 1000mg/1000g everyday oral along with Doxorubicin 0.003g/1000g/ at 7TH day intraperitonealy and Do recieved Nigella sativa 1000mg/kg everyday oral. At the end of experiment, rats were dissected and conserved slides for staining.
Results: Bo showed loss of normal architecture of seminiferous tubules and diverse level of atrophy while condition was improved in Co which were given Doxorubicin with extract of Nigella sativa.
Conclusion: Our result tells us that Nigella sativa amended the histomorphological changes.