Open AccessDesign, Synthesis and MAO Inhibitor Activity of Chroman-4-one Derivative
Author(s) -
Somesh Kumar Saxena,
Pathak Ashish,
Om P. Agrawal
Publication year - 2021
Publication title -
journal of pharmaceutical research international
Language(s) - English
Resource type - Journals
ISSN - 2456-9119
DOI - 10.9734/jpri/2021/v33i63a35826
Subject(s) - chemistry , substrate (aquarium) , reagent , quenching (fluorescence) , amine gas treating , electrophile , fluorescence , inhibitory postsynaptic potential , tyramine , amine oxidase , combinatorial chemistry , biochemistry , stereochemistry , organic chemistry , catalysis , biology , neuroscience , ecology , physics , quantum mechanics
Two chalcones (4a, 4b) and nine Schiff bases (4c – 4k) of 7-hydroxy-3-formyl chromen-4-one have been synthesized. All the synthesized compounds 1–18 were screened for their hMAO (human Mono Amine Oxidase) inhibitory activity using recombinant human MAO isoforms. hMAO inhibitory activity was determined by measuring the production of H2O2 from p-tyramine, the common substrate for both hMAO-A and hMAO-B, using the Amplex1-RedMAO assay kit. The compounds and reference inhibitors did not react directly with the Amplex1-Red reagent indicating that they do not interfere with the measurements. The compounds were also confirmed, as they did not interact with resorufin by treating the maximum concentration of compounds with various concentrations of resorufin in order to detect if the fluorescence signal is the same with or without our compounds in the medium. No significant quenching of resorufin was observed.