Open AccessDevelopment and Validation of UV-Visible Spectrophotometric Method for Estimation of Tacrolimus in Bulk and Pharmaceutical Nanoparticles
Author(s) -
Snehal Patel,
Chintan Aundhia,
A. K. Seth,
Nirmal Shah,
Dipti Gohil,
Ghanshyam Parmar,
Sapna Desai,
Kartik Pandya
Publication year - 2021
Publication title -
journal of pharmaceutical research international
Language(s) - English
Resource type - Journals
ISSN - 2456-9119
DOI - 10.9734/jpri/2021/v33i62b35192
Subject(s) - relative standard deviation , tacrolimus , nanoparticle , wavelength , materials science , pharmaceutical formulation , chromatography , chemistry , analytical chemistry (journal) , nanotechnology , optoelectronics , detection limit , medicine , surgery , transplantation
A UV-visible spectrophotometric method for quantifying tacrolimus in nanoparticles has been developed and validated, and it is accurate, simple, reproducible, and affordable. For drug analysis, optimal conditions have been identified. 291 nm was discovered to be the maximum wavelength (max). In the range of 0.2-1.8 mg/ml, the response was linear (r2 = 0.9989). The intra- and inter-day relative standard deviations for precision studies were found to be less than 2%, indicating that the procedure is precise.