Open AccessStudy on the Plasmid and Genomic DNA Stability of DH5α Host System
Author(s) -
Laxmisai Dixitha Vengili,
Phavethra Sago,
Yaswanth Kumar Reddy Kuncha,
V. S. Kumar Kolli,
Lasya Sri Varugu,
Sree Lakshmi Mandadapu,
Suryanarayana Veeravilli,
Praveen Kumar Vemuri
Publication year - 2021
Publication title -
journal of pharmaceutical research international
Language(s) - English
Resource type - Journals
ISSN - 2456-9119
DOI - 10.9734/jpri/2021/v33i60b34751
Subject(s) - plasmid , restriction enzyme , ecori , dna , genomic dna , biology , bamhi , computational biology , genetics
Plasmids are extremely valuable as a source of DNA and for use in biotechnology. Our research goal was to develop plasmids with extremely high copy numbers for lab-scale plasmid preparations. Using the beverage method simplifies the process of obtaining a large quantity of plasmid DNA for various applications. Diet Coke as a beverage demonstrated that DNA recovery is highly adequate. Diet Sting with EcoRI and BamHI enzymes was used to detect restriction digestion with high sensitivity. However, for lab-scale preparative work, our findings show that plasmid yield can be significantly increased by using standard growth procedures and commonly used growth media.