Open AccessSpectroscopic Estimation of Doxylamine Succinate in Tablets and Human Plasma by Formation of Ion-pair Complex
Author(s) -
M. Narender,
Sowjanya Ravuri,
Balakrishna Muthyala,
Narayana Rao Alla,
Anka Rao Areti
Publication year - 2021
Publication title -
journal of pharmaceutical research international
Language(s) - English
Resource type - Journals
ISSN - 2456-9119
DOI - 10.9734/jpri/2021/v33i59a34325
Subject(s) - absorbance , chemistry , analyte , chloroform , chromatography , ion , complex formation , human plasma , buffer solution , analytical chemistry (journal) , inorganic chemistry , organic chemistry
Aim: To develop a simple spectroscopic method for estimation of doxylamine (DOX) succinate in its tablet dosage form and human plasma with the aid of an ion-pair complex formation.
Methods: In this method, methyl orange (0.05 % w/v) dye was used to form an ion-pair complex in acetate buffer (1M; pH: 5.00) at 300 C ± 20C. The ion-pair complex formed was extracted with chloroform. The maximum absorbance for the ion-pair complex was measured at 420 nm.
Results and discussion: The method conditions were obeyed Beer's law in the concentrations ranging from 5-25 µg/mL of DOX succinate with a correlation coefficient (r2) of 0.992. The ion-pair (drug-dye) complex was formed in a 1:1 ratio which was demonstrated by Jobs' method of continuous variation. The method was satisfied the validation criteria as per ICH (Q2R1) guidelines. Accuracy studies showed 99.06-100.9 % recovery of the analyte. The responses of the precision and robustness were found within acceptable limits (<2% RSD). The LOD and LOQ values were found as 0.31 and 0.939 µg/mL, respectively.
Conclusion: The developed method was simple, specific, and economical and requires a short analysis time. Therefore it could be considered for precise analysis of DOX succinate in biological matrices.