Open AccessStability of Insulin on Polycaprolactone Nanoparticles as a Function of Surface Properties
Author(s) -
Nader Ibrahim Namazi
Publication year - 2021
Publication title -
journal of pharmaceutical research international
Language(s) - English
Resource type - Journals
ISSN - 2456-9119
DOI - 10.9734/jpri/2021/v33i56b33956
Subject(s) - isoelectric point , nanoparticle , dynamic light scattering , polycaprolactone , adsorption , chemical engineering , materials science , scanning electron microscope , fluorescence spectroscopy , fluorescence , morphology (biology) , insulin , particle size , particle (ecology) , chemistry , chromatography , nanotechnology , polymer , organic chemistry , optics , composite material , enzyme , endocrinology , oceanography , engineering , biology , genetics , medicine , physics , geology
The purpose of this research was to formulate insulin-loaded polycaprolactone (PCL) nanoparticles and evaluate structural stability of the protein using fluorescence spectroscopy. The size and morphology of the nanoparticles were characterized using dynamic light scattering (DLS) and scanning electron microscopy (SEM). Fluorescence emission data revealed that insulin is most stable with multilayer adsorption at pH close to its isoelectric point (IEP). The obtained particle size ranged from 130-140 nm+22 (SD). The loading amount of insulin onto the PCL nanoparticles was low at pH 7.4 and relatively high at pH 5.3. The adsorption phenomenon of protein onto hydrophobic nanoparticles provides a promising noninvasive carrier system for insulin.