Open AccessPharmacokinetic and Phytochemical Appraisal of Trigonella foenum and Andrographis lineata in Dimethylnitrosamine Induced Injury in Albino Rats
Author(s) -
Sara Zahid,
Asma Ahmed,
Hasan Akbar Khan,
Fatima Zahid,
Zeeshan Akbar,
Hafsa Waheed,
Ambreen Abbas
Publication year - 2021
Publication title -
journal of pharmaceutical research international
Language(s) - English
Resource type - Journals
ISSN - 2456-9119
DOI - 10.9734/jpri/2021/v33i46b32917
Subject(s) - trigonella , phytochemical , chemistry , ascorbic acid , dpph , ethyl acetate , antioxidant , andrographis paniculata , traditional medicine , andrographolide , piperine , pharmacology , biochemistry , food science , organic chemistry , medicine , alternative medicine , pathology
Trigonelline is a plant alkaloid and Andrographolide is a diterpene lactone, both exhibiting anti-inflammatory, antioxidant and neuroprotective activities. The present study was designed to evaluate the antioxidative and anti-inflammatory activity of the above said compounds in Dimethylnitrosamine induced toxicity in albino rats. Extraction of Trigonella foenum and Andrographis lineata was carried out by using methanol, petroleum ether, ethyl acetate and ethanol assisted with suitable temperature, followed by DPPH scavenging activity (IC-50) of these extracts.Cmax, Tmax, t1/2, CL, Vd and AUC were evaluated as pharmacokinetic parameters by using calibration curves of Andrographolide and Trigonelline. Extracts of T. foenum-graecum and A. lineate have antioxidant activity by inhibiting DPPH (IC50 value was 69.04±3.65% and 71.76±6.99%, respectively) comparable with ascorbic acid (53.99±4.88%). Phytochemical analysis of T. foenum-graecum and A. lineate was found with maximum number of phenols in them, with least recovery in the ethyl acetate extract while maximum phenols were found in T. foenum graecum. All the pharmacokinetic parameters of trigonelline and andrographolide administered in low dose (200 mg/kg and 50 mg/kg respectively) confirmed the better antioxidative activity than that of ascorbic acid used as a potent antioxidant.