Open AccessFabrication of Chitosan Based Antibacterial Implant for Multibacterial Bone Infection
Author(s) -
Ashish Pawar,
Sejal R. Jadhav,
Deelip V. Derle
Publication year - 2021
Publication title -
journal of pharmaceutical research international
Language(s) - English
Resource type - Journals
ISSN - 2456-9119
DOI - 10.9734/jpri/2021/v33i46a32861
Subject(s) - chitosan , epichlorohydrin , ciprofloxacin , osteomyelitis , ciprofloxacin hydrochloride , in vivo , antibiotics , tetracycline hydrochloride , minimum inhibitory concentration , bone infection , drug , implant , biomedical engineering , chemistry , materials science , pharmacology , medicine , surgery , organic chemistry , biology , tetracycline , biochemistry , microbiology and biotechnology
Despite advancements in medicines and novel surgical methods, osteomyelitis is still a complex and difficult to treat multi-bacterial bone infection. It generates in tissue necrosis along with rupturing of bone in chronic cases leading towards limited vascularity at infection site, local treatment might not show accountable level of antibiotic at infection site. The present research aims towards development and evaluation of bio-degradable implant of Ciprofloxacin hydrochloride to treat local osteomyelitis. Chitosan is widely investigated biodegradable polymer with hydroxyl moiety which is active and can be altered chemically to develop biomedical and therapeutic dosage forms. Here this active group was modified using epichlorohydrin & crosslinked Chitosan matrices were used as carrier to formulate different Ciprofloxacin implant. In vivo parameters and in vitro study was conducted for optimized batch. The formulation having 40% drug loading (EC4) was found to be optimum when all evaluation parameters were tested. The concentration of Ciprofloxacin Hydrochloride (HCl) in bone and surrounding tissues is much higher than minimum inhibitory concentration (MIC) even after a month. After observing the water uptake and extended release of drug from all formulations the drug loading was found to be higher. The present work concluded that, the highly branched chitosan remarkably reduce the percent (%) drug release up to a month which could be useful in the treatment of osteomyelitis.