Open AccessGoat’s Milk as a Potential Anti-proliferative against Colon Cancer Cell Lines
Author(s) -
Nurhashimah Dahlan,
Jamila Khayrin Baharum,
Norhaslinda Ridzwan,
Mimie Noratiqah Jumli,
Norhayati Abd Hadi,
Roslan Arshad,
Atif Amin Baig,
Mohd Adzim Khalili Rohin,
Che Abdullah Abu Bakar,
Adibah Abdul Latif
Publication year - 2021
Publication title -
journal of pharmaceutical research international
Language(s) - English
Resource type - Journals
ISSN - 2456-9119
DOI - 10.9734/jpri/2021/v33i44a32642
Subject(s) - pasteurization , apoptosis , mtt assay , fragmentation (computing) , food science , ic50 , chemistry , colorectal cancer , cell , microbiology and biotechnology , cancer , biology , in vitro , biochemistry , ecology , genetics
Aim: To investigate anti-proliferative effect of fresh and pasteurized goat milk against colon cancer cell lines (HT-29, HCT-116, CT-26).
Study Design: Experimental study.
Place and Duration of Study: Central Laboratory, Tissue Culture Laboratory, Universiti Sultan Zainal Abidin, Terengganu between January 2020 and April 2020.
Methodology: Samples comprised of two types goat milk, which were fresh and pasteurized in powder form. The samples were analysed for the anti-proliferative effect by MTT assay, and IC50 value was determined. Then, cell apoptotic changes were observed by light inverted microscope by 24, 48 and 72 hours.
Results: Experimental data showed that the fresh sample produce the highest yield (9.40%) than the pasteurized sample (7.17%). The fresh sample yielded the most potent cytotoxic value (0.28 ± 0.03), followed by pasteurized sample with value IC50 0.32 ± 0.02 against HCT-116 cells. Then, the anti-proliferative effect was observed on cell apoptotic changes by reduction of cell volume, cell densed, and presence of fragmentation and apoptotic bodies at 24, 48 and 72 hours treatment.
Conclusion: In conclusion, the fresh sample of goat milk yielded the potent anti-proliferative effect than pasteurized sample.