Open AccessChemometric Access for RP-HPLC Simultaneous Estimation of Tadalafil and Dapoxetine Applying Response Surface Methodology
Author(s) -
Sabarinath Jayaseelan,
N. Kannappan,
V. Ganesan
Publication year - 2021
Publication title -
journal of pharmaceutical research international
Language(s) - English
Resource type - Journals
ISSN - 2456-9119
DOI - 10.9734/jpri/2021/v33i43b32553
Subject(s) - tadalafil , response surface methodology , analyte , chromatography , central composite design , design of experiments , high performance liquid chromatography , volumetric flow rate , resolution (logic) , phosphate buffered saline , retention time , chemistry , analytical chemistry (journal) , computer science , mathematics , statistics , artificial intelligence , medicine , physics , quantum mechanics , sildenafil
Aims: A RP-HPLC method was developed and validated for simultaneous estimation of Tadalafil and Dapoxetine applying statistical experimental design.
Methodology: Multivariate optimization of the experimental conditions of RP-HPLC method was using Design of experiments. Independent three factors like phosphate buffer pH, mobile phase composition and flow rate were applied to design mathematical models. To study the response surface methodology by using Central composite design (CCD). In depth the effects of these independent factors was studied using CCD. Simultaneously optimize the retention time and resolution of the analytes was applying Desirability function.
Results: The predicted and optimized data from contour picture containing phosphate buffer (pH 3.4) and acetonitrile in the ratio of 40:60%v/v respectively. Flow rate was found to be 0.8 ml/min. Baseline separation of both analytes with run time of less than 10.0 min and good resolution were achieved using these optimum conditions.
Conclusion: Method was validated according to ICH guidelines by using optimized assay conditions. Therefore, the reports distinctly indicated that Quality by design access could be satisfactorily used to optimize RP-HPLC method for simultaneous estimation of Tadalafil and Dapoxetine.