Open AccessA Simple LC-MS/MS Method for Determination of Vemurafenib in Rat Plasma Fed with High Fat Diet
Author(s) -
Ayşe Özdemir,
Senem Şanlı,
Erten Akbel
Publication year - 2021
Publication title -
journal of pharmaceutical research international
Language(s) - English
Resource type - Journals
ISSN - 2456-9119
DOI - 10.9734/jpri/2021/v33i40b32285
Subject(s) - detection limit , chromatography , vemurafenib , calibration curve , chemistry , mass spectrometry , liquid chromatography–mass spectrometry , tandem mass spectrometry , medicine , cancer , metastatic melanoma
In this study, high fat diet was fed to rats and the amount of vemurafenib in rat plasma was determined by the developed liquid chromatography with tandem mass spectrometry. The calibration curve was linear between 0.01 and 0.8 µg mL−1 vemurafenib with 0.999 regression coefficient. The limit of detection and quantification of the method are estimated from the signal to noise ratio 3:1 and 10:1, respectively. These are 1.10-4 µg mL−1 for LOD and 4. 10-4 µg mL−1 for LOQ. This method has been found to be reproducible and highly sensitive and provides a combination of faster analysis time and improved limits of detection.