Open AccessBiotechnology of Microorganisms Growing– Fundamentals for the Development of a Litter Biodestructor
Author(s) -
Анна Николаевна Гнеуш,
Albina Vladimirovna Luneva,
Надежда Леонидовна Мачнева,
Yury Lysenko,
M. V. Aniskina,
Mariikolaevna Verevkina,
Magomed Hizrievich Kilyashanov,
Sergey Nikolaevich Povetkin
Publication year - 2021
Publication title -
journal of pharmaceutical research international
Language(s) - English
Resource type - Journals
ISSN - 2456-9119
DOI - 10.9734/jpri/2021/v33i36b31946
Subject(s) - agitator , microorganism , aeration , bioreactor , pulp and paper industry , pseudomonas , chemistry , ammonia , food science , microbiology and biotechnology , biology , botany , chromatography , biochemistry , bacteria , ecology , mass transfer , engineering , genetics
The purpose of the research work was to select the optimal conditions for the cultivation of microorganisms. As a result of the conducted research work, the modes of growing a nitrogen-fixing culture and a microorganism with high enzymatic activity were selected and worked out. At the same time, the optimal conditions for the cultivation of Azotobacter sp were determined – the temperature optimum for cell accumulation was 30°C, for increased polysaccharide production 20 °C, aeration within 5-10 l/l/min, agitator speed-150 rpm, pH value within 6.0±0.2 units, which allowed to achieve a cell titer of at least 1.0×109 CFU/ml. A cost-effective nutrient medium was selected for growing Pseudomonas sp. molasses-autolysate medium and optimal conditions for growing the culture: cultivation temperature 30-32 °C, aeration 1.0-1.5 l/l/ min, agitator speed 150-200 rpm, pH 6.8-7.2 units, sub-titration 5.0 % KOH, defoaming with adecanol, cultivation time-72 hours, which allowed to achieve a cell titer of at least 1.0×109 CFU/ml.