Open AccessStatistical Optimization of Temperature, Concentration, RPM and pH for the Surface Tension of Biosurfactant by Achromobacter Xylos GSR21
Author(s) -
Balaji Somesam Upadhyaya,
G. Venkata Ramana Reddy,
Mallu Maheshwara Reddy,
Sohom Adhikari,
Shashank Rajkumar,
Akula Niranjan Babu,
Vanga Manav Goud,
Chelikani Sidhartha,
Varakala Nikhil Reddy,
Divyanshu Dhakate,
N. Konda Reddy
Publication year - 2021
Publication title -
journal of pharmaceutical research international
Language(s) - English
Resource type - Journals
ISSN - 2456-9119
DOI - 10.9734/jpri/2021/v33i331161
Subject(s) - response surface methodology , pulmonary surfactant , surface tension , achromobacter , central composite design , petrochemical , chemistry , strain (injury) , chemical engineering , materials science , chromatography , thermodynamics , organic chemistry , engineering , geology , biology , biochemistry , pseudomonas , paleontology , physics , anatomy , bacteria
Achromobacter xylos strain GSR21 plays a crucial role in bioremediation of fossil fuel contamination, biopharmaceutical, cosmetics, chemical, petroleum refining, petrochemical, food industries and tertiary oil recovery (Microbial enhanced oil recovery). Response surface quadratic models (RSQM) was applied to reinforce the censorious operating conditions for the assembly of Achromobacter xylos strain GSR21. The Response surface method (RSM) was application to determine the best degrees of cycle factors (Temperature, Concentration, RPM, pH). Central composite design (CCD) of RSM was used to contemplate the four factors at five levels, and strain GSR21 Achromobacter xylos fixation was approximate as a reaction. Relapse coefficients predicted by examination and therefore the model was settled. R2 value regard for bio-surfactant (mN/m) attempted to be 0.81, showing that the model fitted well with the explorative results. The mathematical model predicted by simulation of the foreseen updated values, and bio-surfactant surface tension was found 50 mN/m. The foreseen model was matched at 98.8% with the test outcomes coordinated under the perfect conditions. Based on the finding research, temperature-40°C, concentration-1.8 g/l, RPM-180 rev/mint and pH-4 was perceived as compelling fragments for Achromobacter xylos GSR21.