Open AccessEffect of Olea europaea L. and Juglans regia L. Extracts on Human Cancer Cell Line Viability with Studying of Hypoglycemic and Antiglycation Properties
Author(s) -
Abdulkarim Dakah,
Raida Khalil,
Chahed Al Masri,
Marwa Al Matar,
Saba Hameed,
Huzaifa Yousif,
Eman Albsoul,
Tala Issa
Publication year - 2021
Publication title -
journal of pharmaceutical research international
Language(s) - English
Resource type - Journals
ISSN - 2456-9119
DOI - 10.9734/jpri/2021/v33i1431269
Subject(s) - olea , juglans , chemistry , hela , viability assay , traditional medicine , mtt assay , cytotoxicity , food science , biochemistry , botany , biology , cell , medicine , in vitro
There is an urgent and continuous need to discover new sources of medicinal plants to obtain useful compounds with health properties. So the purpose of this study was to investigate activity of Olea europaea L and Juglans regia leaves extract on Hela cell line viability, antidiabetic and Antiglycation. The aqueous extracts were obtained from leaves. Alloxan 180 mg /kg body was used to induce diabetes. Mice with blood glucose level of ≥200 mg/dl were considered as diabetic and were received 10 mg/kg of body weight of Extracts. For Antiglycation, SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis) were prepared for the appearance of high molecular weight products. Hela cell line were cultured in RPMI-1640 medium for MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay analysis. The results showed that the mean of glucose levels decreasing in mice that treated with 10 mg/Kg extracts of O. europaea and J. regia. Also SDS-PAGE showed that extracts of J. regia were better than of O. europaea in inhibition of glycation induced protein cross-linking at all studied concentrations. MTT assay showed that the Cytotoxicity was increased with increasing the doses of extract and the cytotoxic effects of J.regia extract were higher than O.europaea at all concentrations. This study showed promising results.