Open AccessEthnobotanical Survey and Evaluation of Anti-Salmonella Potentials of Commonly Used Plants for Typhoid Treatment in Ogbomoso, Oyo State, Nigeria
Author(s) -
Olaniyi Temitope Deborah,
Tamilore Modupe Awodugba,
Adewale Adetutu
Publication year - 2021
Publication title -
journal of complementary and alternative medical research
Language(s) - English
Resource type - Journals
ISSN - 2456-6276
DOI - 10.9734/jocamr/2021/v15i130254
Subject(s) - salmonella typhi , traditional medicine , typhoid fever , ciprofloxacin , medicine , agar , maceration (sewage) , widal test , veterinary medicine , biology , microbiology and biotechnology , antibiotics , bacteria , biochemistry , genetics , materials science , escherichia coli , composite material , gene
This study assessed the sensitivity of S. typhi to extracts of commonly used plants in treatment of typhoid in Ogbomoso, Oyo State, Nigeria. Ethnobotanical survey was conducted using a structured questionnaire. Powdered leaves of plants were extracted by maceration in distilled water. In vitro sensitivity of S. typhi to the extracts were assessed using agar well diffusion method. Groups of rats were infected orally with 0.5 McFarland suspension of S. typhi. Bacteremia in the animals was monitored by plating on Salmonella-Shigellar agar. Biochemical parameters were determined spectrophotometrically. Histological analysis was performed using H&E staining method. S. typhi was sensitive only to P. guajava and A. indica leaf extracts. V. amygladina (90.31%) and C. papaya (92.26%) showed highest percentage inhibition of S. typhi comparable with ciprofloxacin (99.86%). Haematological parameters varied significantly (p<0.05) among the groups. Antioxidant status of rats, total protein, albumin and the corresponding globulin level increased significantly (p<0.05) in the groups treated with P. guajava and A. indica. C. papaya and C. citratus ameliorated the histopathological damages observed in the liver and intestine of S. typhi infected rats. The studied plants have direct activities against S. typhi, thus reflecting the reason for their combination in traditional system of medicine.