Open AccessDesign and Development of Mini-pulsar for Real-Time Bioelectric Experiment
Author(s) -
Gyanendra Kumar,
R. Sarathi,
Archana Sharma
Publication year - 2021
Publication title -
journal of engineering research and reports
Language(s) - English
Resource type - Journals
ISSN - 2582-2926
DOI - 10.9734/jerr/2021/v20i617322
Subject(s) - nanosecond , microsecond , electric field , electroporation , blumlein pair , rise time , optoelectronics , pulsed power , materials science , electrical engineering , voltage , physics , nanotechnology , optics , chemistry , pulse generator , laser , engineering , biochemistry , quantum mechanics , gene
Investigation of the effect of pulsed electric field on eukaryotic cells has been of interestto biomedical scientists and researchers since a few decades. Reversible electroporation (EP) is used for uptake of chemicals, drugs, DNA into the eukaryotic cells under low electric field (100’s V/cm) of millisecond duration to few hundreds of microsecond duration. An electric field of nanosecond duration and a very high electric field (50’s kV/cm) can stress to intracellular organelles of eukaryotic cells and that can trigger apoptotic pathways. In this article experimental setup has been prepared for real-time investigation of the effect of nanosecond electric field on eukaryotic cells. Pulsar producing ~1.5kV, 20ns FWHM, single shot as well as ~10Hz rep-rate with rise-time as fast as ~10ns, has been prepared using blumlein pulse forming line and avalanche transistor switches. A 300µm gap microplate chamber has been prepared to expose the electric fieldtoeukaryotic cells.