Open AccessIsolation of Cercospora canescens and Management of Cercospora Leaf Spot (Cercospora canescens) of Mothbean through Botanicals
Author(s) -
Rakesh Kumar,
Anand Kumar Meena,
Vikash Kumar,
Jasveer Singh
Publication year - 2022
Publication title -
journal of experimental agriculture international
Language(s) - English
Resource type - Journals
ISSN - 2457-0591
DOI - 10.9734/jeai/2022/v44i530824
Subject(s) - cercospora , leaf spot , mycelium , biology , conidium , potato dextrose agar , botany , sclerotium , spots , horticulture , agar , genetics , bacteria
The pathogen was isolated from diseased leaves of mothbean on potato dextrose agar by standard tissue isolation method and pathogenicity was proved following Koch's postulates. On the basis of morphological studies like mycelium was whitish to brownish, hyaline and acircular, straight to variously curved, multiseptate (2-16) many celled, thread like conidia and cultural studies the pathogen was identified as Cercospora canescens Ellis and Martin. Among eight botanicals tested at three concentrations (5, 10 and 15%) under in vitro condition against C. canescens, neem seed kernel extract was found most effective and inhibiting mycelial growth (58.00%) at 15% concentration followed by tulsi leaf extract (52.96%) and neem leaf extract (44.68%) also found effective. Least mycelial growth inhibition of C. canescens was obtained in turmeric extract (27.31%) and giloy (25.31%) extract at 15% concentration.