Open AccessOccurrence of Aflatoxins in Rice Intended for Infant Flour Production in Ouagadougou, Burkina Faso
Author(s) -
Hamidou Compaoré,
Serge Samandoulougou,
Clarisse S. Compaoré,
Alima Bambara,
Hissein Ratongué,
L. Traoré,
Hagrétou Sawadogo-Lingani
Publication year - 2021
Publication title -
journal of advances in microbiology
Language(s) - English
Resource type - Journals
ISSN - 2456-7116
DOI - 10.9734/jamb/2021/v21i1030393
Subject(s) - aflatoxin , aspergillus flavus , aspergillus , aspergillus parasiticus , food science , contamination , agar , mycotoxin , biology , incubation , mold , veterinary medicine , chemistry , microbiology and biotechnology , botany , medicine , bacteria , ecology , biochemistry , genetics
A total of four samples of rice intended for infant flour production in Ouagadougou were received at the Physico-chemistry laboratory of Food Technology Department (DTA) for quality control. The latter were also tested for Aspergillus section Flavi presence and analyzed for aflatoxins B1, B2, G1 and G2 content using high performance liquid chromatography (HPLC). Among the twenty (20) strains of mold isolated from these samples, three Aspergillus section Flavi were obtained and cultivated in “Aspergillus flavus and parasiticus Agar (AFPA)” to ascertain if they belong to Aspergillus flavus or Aspergillus parasiticus species. The qualitative ability of aflatoxin production was also performed by fluorescence emission under ultra violet light at 365 nm after four days of incubation at 30 °C on Coconut Agar Medium (CAM). Statistical analysis results showed that 75% of samples were contaminated with total aflatoxins (AFs) with contents ranging from 0.54 ± 0.06 to 2.40 ± 0.07 µg/Kg. Aflatoxin B1 (AFB1) and aflatoxin B2 (AFB2) were detected in two contaminated samples. AFB1 had the highest concentration as compared with other aflatoxins. A significant level of contamination (p< 0.0001) was observed in sample R441 compared to other sample types.