Open AccessChanges in Antioxidant Markers and Histopathology of Wistar Rats Fed with Edible Clay (Nzu)
Author(s) -
A. Dokubo,
A. E. Okwudike,
K. T. Nwauche
Publication year - 2021
Publication title -
journal of applied life sciences international
Language(s) - English
Resource type - Journals
ISSN - 2394-1103
DOI - 10.9734/jalsi/2021/v24i130216
Subject(s) - histopathology , catalase , superoxide dismutase , antioxidant , glutathione , reactive oxygen species , oxidative stress , chemistry , significant difference , dismutase , malondialdehyde , medicine , biology , zoology , endocrinology , food science , biochemistry , pathology , enzyme
The changes in the antioxidant markers and histopathology in both adult male and female wistar rats fed with Calabash Chalk (nzu) was investigated. Twenty (20) wistar rats weighing between (120-150 g) were used for this study. They were randomly divided into four (4) Groups containing five (5) animals each (n=5) A-D. Groups (B-D) were fed with 1.0%, 2.0% and 8.0% of clay. Group A, not fed with clay served as control. After 21 days of continuous feeding, the animals were sacrificed and their liver organs excised for the following antioxidant markers (Catalase CAT, Reduced Glutathione GSH, Superoxide dismutase, SOD) and histopathology. The results showed significant (p 0.05) difference was obtained for the group fed with 2.0% when compared to control. The results obtained for GSH and SOD also showed no significant (p>0.05) difference in the fed groups when compared to control. Histopathological changes indicated mild periportal and intraparenchymal inflammation in group fed with 8.0%. From the study it can be deduced that clay consumption has the potential to elicit the activities of antioxidant markers and subsequent depletion providing weak defenses against reactive oxygen species (ROS) and liver damage in the rats.