Open AccessInfluence of Deep Eutectic Solvents (DESs) on Antioxidant and Antimicrobial Activity of Seed Extracts of Selected Citrus Species
Author(s) -
Lamija Kolarević,
Emir Horozić,
Zahida Ademović,
Broza Šarić Kundalić,
Darja Husejnagić
Publication year - 2020
Publication title -
international research journal of pure and applied chemistry
Language(s) - English
Resource type - Journals
ISSN - 2231-3443
DOI - 10.9734/irjpac/2020/v21i2330309
Subject(s) - chemistry , choline chloride , deep eutectic solvent , dpph , antimicrobial , antioxidant , food science , lactic acid , chloride , ferric , antibacterial activity , nuclear chemistry , organic chemistry , eutectic system , bacteria , alloy , biology , genetics
Deep eutectic solvents (DESs), as an alternative to ionic liquids, have greener credentials than ionic liquids and have attracted increasing attention in many applications. Choline chloride-based DESs in combination with different hydrogen bond donors (organic acid, sugars and urea), showed a high extraction efficiency. The aim of this study was to examine the possible effects of choline chloride-based eutectic solvents on the antioxidant and antimicrobial activity of lemon and mandarin orange seed extracts. Lactic acid, glucose, urea and water were used to prepare choline chloride-based Natural Deep Eutectic Solvents (NADESs). Ferric-Reducing Antioxidant Power (FRAP) Assay and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) method were used to determine antioxidant activity. Antibacterial activity was investigated by diffusion method on reference bacterial strains E. coli, E. faecalis, S. aureus, B. subtilis and L. monocytogenes. The analysis revealed a significant reduction potential of the eutectic solvent based on lactic acid as well as significant antioxidant activity of lemon and mandarin seed extracts. In general, mandarin extracts showed better antioxidant capacity. In vitro antibacterial activity assays showed a complete absence of bacterial growth inhibition of the extracts. However, eutectic solvents with lactic acid have shown a significant antimicrobial effect.