Open AccessFirst Report in Sudan: Detection of Antibacterial Activity of the Black Cumin (Nigella sativa) Seed Extract against Mycoplasma mycoides subsp mycoides (Mmm)
Author(s) -
Raghad A. H. Onsa,
E. A. Muna,
Samia A. A. Hassan
Publication year - 2019
Publication title -
international journal of pathogen research
Language(s) - English
Resource type - Journals
ISSN - 2582-3876
DOI - 10.9734/ijpr/2019/v2i129622
Subject(s) - nigella sativa , mycoplasma mycoides , serial dilution , biology , strain (injury) , traditional medicine , veterinary medicine , microbiology and biotechnology , mycoplasma , medicine , alternative medicine , pathology , anatomy
Aims: This study was focused on the effect of Nigella sativa seed oil on the in vitro growth of Mycoplasma mycoides subsp mycoides (Mmm).
Study Design: Three strains of (Mmm) were subjected to different dilutions of extracted Nigella sativa seed oil and the inhibitory zones were recorded. Type of effect (bactericidal or bacteriostatic) was studied.
Place and Duration of Study: The study was carried out at Mycoplasma and biochemistry departments Central Veterinary Research Laboratory (CVRL) - 2018.
Methodology: The tested strains were: Reference strain (T1/44), recent local strain (RH strain) and the last one was an old local strain (F strain). Different dilutions were used from N. sativa (25%, 50%, 75% and 100%) respectively.
Results: The N. sativa revealed different inhibition zones on the growth of the three mentioned cultures. In addition to that bactericidal effect on (Mmm) growth was observed.
Conclusion: The result of this study suggests the potential use of Cumin seed (N. sativa) against (Mmm) in vitro, and this result contributes in CBPP disease control using this type of natural seeds.