Open AccessAn Effective Protocol for Carnation (Dianthus caryophyllus L.) cv. 'Geolei' Explants Sterilization for Successful Callusing and Shoot Regeneration
Author(s) -
Ujjwal Sirohi,
Swati Sharma,
Mukesh Kumar,
R. S. Sengar,
L. K. Gangwar,
Akash Tomar,
Vyankatesh Dhanraj Bagul,
Manoj Kumar Yadav
Publication year - 2021
Publication title -
international journal of environment and climate change
Language(s) - English
Resource type - Journals
ISSN - 2581-8627
DOI - 10.9734/ijecc/2021/v11i1130538
Subject(s) - explant culture , sodium hypochlorite , shoot , carnation , horticulture , sterilization (economics) , callus , biology , dianthus , murashige and skoog medium , botany , chemistry , in vitro , biochemistry , organic chemistry , economics , monetary economics , foreign exchange market , foreign exchange
Carnation is a popular floricultural crop grown widely for its attractive cut flowers. Micro-propagation can be used to create large-scale carnation output. For growth and development, plants require some necessary nutrients as well as growth regulators. Due to the importance of carnation, the present work is carried out using leaf and nodal segments to examine the potential of several plant growth regulators for in vitro callus formation and adventitious shoot regeneration. Explants were sterilized properly with bavistin, sodium hypochlorite and mercuric chloride. The minor contaminated cultures were created by consecutively treating the explants with 0.25% bavistin, 0.50% sodium hypochlorite, and 0.1% mercuric chloride for ten, fifteen, and two minutes.
MS media with 2.5 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) in combination with 0.75 mg/l naphthalene acetic acid (NAA) resulted in the maximum callus induction (90.47%) from leaf explants. Maximum shoots (76.47%) were produced in MS media supplemented with 2.0 mg/l Thidiazuron (TDZ) + 0.25 mg/l NAA. NAA at 1.25 mg/l was most efficient for maximum root induction (83.32%). In the present study, an effective protocol of carnation explants sterilization was optimized for successful callusing and shoot regeneration.