Open AccessCytotoxicity and Genotoxicity of Sunset Yellow and Potassium Sorbate in Jurkat Cell Line
Author(s) -
D. Halilčević,
Esmeralda Dautović,
Melisa Lelić,
Maida Šljivić Husejnović,
Aida Smajlović,
Nahida Srabović,
Adaleta Softič
Publication year - 2022
Publication title -
international journal of biochemistry research and review
Language(s) - English
Resource type - Journals
ISSN - 2231-086X
DOI - 10.9734/ijbcrr/2022/v31i230301
Subject(s) - jurkat cells , genotoxicity , cytotoxic t cell , micronucleus test , potassium sorbate , micronucleus , cytotoxicity , chemistry , cell culture , dna damage , viability assay , apoptosis , biology , in vitro , biochemistry , immunology , toxicity , t cell , dna , genetics , immune system , sugar , organic chemistry
Aims: Industrial processed food, pharmaceutical and cosmetical products contain numerous substances the safety of which has been widely concerned. Potassium sorbate (PS) is a common preservative used in a wide-range of products. Sunset yellow (SY) is one of the most commonly used dye in food and pharmaceutical industry. Several studies suggested cytotoxic and genotoxic potential of SY and PS in different cell lines. These effects can lead to organ damage and cancer development. The aim of this study is to investigate cytotoxic and genotoxic potential of two widely used food additives, SY and PS, in Jurkat cell line.
Methodology: PrestoBlue assay was used to assess the cytotoxic potential of SY and PS. For observation of DNA damage, cytostatic and cytotoxic effects, the cytokinesis-block micronucleus cytome (CBMN) assay was performed.
Results: Decrease of cell viability in Jurkat cell line was observed after 24-hour exposure to both SY and PS. CBMN assay has revealed significant increase of necrotic cells (P<0.05). Genotoxic biomarkers were in physiological range after 24-hour exposure to both analyzed additives.
Conclusion: Our findings suggest that SY, as well as PS have cytotoxic potential in Jurkat cell line, as a result of increased number of necrotic cells. Higher cytotoxic effect was caused by SY compared to PS. However, genotoxic potential was not recorded for any of the food additives analyzed.