Open AccessEffects of Sub-chronic Administration of Crude Ethanol Extract of Cola nitida on Stomach Mucosa Epithelial Lining and Liver Function Enzymes in Albino Rats
Author(s) -
A. Lawal,
M. G. Abubakar,
Abdulkadir Abubakar,
Sadiq Mas’ud Eneji,
Sulaiman Rabiu,
Ebuka Okafor,
U Wali
Publication year - 2022
Publication title -
international journal of biochemistry research and review
Language(s) - English
Resource type - Journals
ISSN - 2231-086X
DOI - 10.9734/ijbcrr/2022/v31i130296
Subject(s) - alkaline phosphatase , cola (plant) , aspartate transaminase , ethanol , alanine transaminase , gastric mucosa , stomach , chemistry , medicine , transaminase , oral administration , enzyme , liver function , endocrinology , pharmacology , biochemistry
Aim: This study investigated the effects of sub-chronic administration of crude ethanol extract of Cola nitida mucosa epithelial lining and liver function enzymes in albino rats.
Place and Duration of Study: Department of Biochemistry and Molecular Biology and Chemical Pathology Usmanu Danfodiyo University, Sokoto, between March 2017 and January 2018.
Methodology: Twenty (20) albino rats have randomly divided into five (5) groups of A, B, C, D, and E of four rats each and were fed with an equal volume of ethanol extract of Cola nitida of 600, 1200, 1800 and 2400 mg/kg body weight of the rats by oral administration for 28 days, while group A serves as a control, respectively.
Results: Result indicates the LD50 was above 5000 mg/kg B.W., serum aspartate transaminase (AST) and alanine transaminase (ALT) activity revealed a significant increase (P = .05) for AST (31.23 ± 9.39), (40.44 ± 12.24), (44.59 ± 8.69), and (36.30 ± 13.18) in rats fed with 600, 1200, 1800 and 2400 mg/kg, and ALT (33.66 ± 7.94) for group fed with 2400 mg/kg B.W as compared against the control group (16.97 ± 6.58) and (20.11 ± 4.39). The serum alkaline phosphatase (ALP) also showed a significant increase (P = .05) (7.99 ± 2.89), (7.07 ± 2.21), and (5.49 ± 1.28) in the group fed with 1200, 1800, and 2400 mg/kg as compared to the control (2.34 ±0.84). Histological studies on the mucosa epithelial lining showed an eroded epithelium and vacuolations at a dose above 1200 mg/kg as compared to the control group.
Conclusion: This study was able to establish that crude ethanol extract of Cola nitida have some deleterious effects on mucosa epithelial lining, and liver function enzymes.