Open AccessToxicological Study of Leaf Extracts of Loranthus micranthus Linn Using Albino Wistar Rats
Author(s) -
Ani Onuabuchi Nnenna,
Ani Okwudili,
Okwuosa Chukwugozie Nwachukwu
Publication year - 2020
Publication title -
international journal of biochemistry research and review
Language(s) - English
Resource type - Journals
ISSN - 2231-086X
DOI - 10.9734/ijbcrr/2020/v29i330175
Subject(s) - alkaline phosphatase , saline , toxicity , bilirubin , urea , chemistry , creatine kinase , acute toxicity , pharmacology , traditional medicine , medicine , biochemistry , enzyme , organic chemistry
This study was aimed to investigate the effect of high sub-chronic doses of the aqueous and methanol leaf extracts of Loranthus micranthus on biochemical parameters of albino rats. Acute toxicity studies were performed according to standard methods. The animals were divided into 5 groups (n = 5). Aqueous and methanol extracts of L. micranthus leaves were administered in doses of 1000 and 2000 mg/kg body weight to four groups of rats respectively for 30 days through the intraperitoneal route. The fifth group served as control and received saline (5 ml/kg b.w, i.p). Blood samples were collected by retrorbital puncture and analyzed for biochemical and haematological parameters using assay kits. Acute toxicity studies indicated that both extracts had an LD50 > 5000 mg/kg. The results indicated significant (p 0.05). There were significant increase in the serum levels of urea in the extracts treated rats (p 0.05). From these results, the extracts caused significant biochemical changes but were not cytotoxic to leucocyte cell lines. Therefore, there should be caution in the long term use of these extracts.