Open AccessChemical Composition and Pharmacological Activity of a Propolis Extract from Ecuador
Author(s) -
Susana Gorzalczany,
Ingrid Márquez Hernandez,
Valeria Sánchez Elizalde,
Mercedes Campo Fernández,
Osmany CuestaRubio,
Rodny Montes de Oca Porto
Publication year - 2022
Publication title -
european journal of medicinal plants
Language(s) - English
Resource type - Journals
ISSN - 2231-0894
DOI - 10.9734/ejmp/2022/v33i330454
Subject(s) - propolis , terpene , triterpene , chemistry , acetic acid , phytochemical , cycloartenol , traditional medicine , chemical composition , gas chromatography–mass spectrometry , anti inflammatory , food science , chromatography , pharmacology , mass spectrometry , biochemistry , biology , organic chemistry , medicine , alternative medicine , sterol , pathology , cholesterol
Aims: Propolis is a resinous substance accumulated by bees from resinous plants material, produced by different botanical processes. It has been used since ancient times for its therapeutic benefits. The chemical composition of propolis is mostly influenced by the geographic zone and also by botanic sources that the honey bee has used. In this sense, the aim of this study was to analyse the phytochemical profile and pharmacological activity of a sample of propolis from Ecuador.
Methodology: Chemical composition was analyzed by using gas chromatography coupled to mass spectrometry (GC/MS). The anti-inflammatory activity was determined by ear edema induced by 12-O-tetradecanoylphorbol-13 acetate and antinociceptive activity was analyzed by using writhing test induced by acetic acid.
Results: The chemical composition showed the presence of sugars, fatty acids, flavonoids and triterpenes in the sample. The analysis of the relative abundance of the detected signals suggested that triterpenes represent 25.38% of the total components of the mixture and 9,19-cyclo-9-beta-lanostane-24-on-3-beta-iloxyl (cycloartenol), the majority compound. Propolis induced a maximun inhibition (77.2 %) in TPA model at a dose of 3 mg/ear, reducing dermal edema, cellular infiltration and ear thickness induced by TPA. Also, it produced a dose-related inhibition of acetic acid-induced writhing response with a maximal antinociceptive effect (49.5%) at dose of 500 mg/kg po.
Conclusion: The chemical analysis showed a predominant triterpene profile, being cycloartenol the majority compound in the sample and its anti-inflammatory and antinociceptive activities were confirmed by in vivo models.