Open AccessEvaluation of the Leaf Extracts of Kalanchoe pinnata and Kalanchoe daigremontiana Chemistry, Antioxidant and Anti-inflammatory Activity
Author(s) -
Edmond J. Quintero,
Estela Guerrero De León,
Juan Morán-Pinzón,
Aldahir Mero,
Edwin León,
Laura Patricia Patiño Cano
Publication year - 2021
Publication title -
european journal of medicinal plants
Language(s) - English
Resource type - Journals
ISSN - 2231-0894
DOI - 10.9734/ejmp/2021/v32i530392
Subject(s) - kalanchoe , dpph , phytochemical , antioxidant , chemistry , traditional medicine , glycoside , petroleum ether , crassulaceae , botany , biochemistry , biology , organic chemistry , extraction (chemistry) , medicine
The aim of this study was to analyze the chemical components and evaluate the biological activity of the extracts from the leaves Kalanchoe pinnata and Kalanchoe daigremontiana, which are cultivated in the province of Chiriqui, Republic of Panama. Phytochemicals components, antioxidant and anti-inflammatory activities were studied. The composition of the obtained petroleum ether, ethanol and aqueous extracts was analyzed by phytochemical screening. The antioxidant activity of the extracts was studied using three in vitro model systems (DPPH radical scavenging assay, nitric oxide radical scavenging assay, and superoxide radical scavenging activity). The anti-inflammatory activity of these species was studied using an in vivo model (ʎ-carrageenan-induced paw edema in rats). Phytochemical analysis of the extracts showed the presence of alkaloids, steroids, triterpenes, flavonoids, phenolic compounds, saponins and glycosides. The greatest radical inhibitory effect was observed in the DPPH model where the ethanolic extracts of both species developed a concentration-dependent inhibitory effect, the K. pinnata extract reached a maximum inhibitory effect of 49.5 ± 5.6% (2000