Open AccessIn-vitro Anti-lipoxygenase and Membrane Stabilization Activity of Crude Extracts of Euphorbia lateriflora (Schum and Thonn)
Author(s) -
A Olasunkanmi Adedoyin,
Olumide Samuel Fadahunsi,
Olorunnisola Olubukola Sinbad
Publication year - 2020
Publication title -
european journal of medicinal plants
Language(s) - English
Resource type - Journals
ISSN - 2231-0894
DOI - 10.9734/ejmp/2020/v31i730251
Subject(s) - chemistry , lipoxygenase , ethanol , ic50 , in vitro , arachidonate 5 lipoxygenase , aqueous solution , euphorbia , chromatography , membrane , pharmacology , traditional medicine , biochemistry , enzyme , biology , medicine , organic chemistry , arachidonic acid
Aims: This study was aimed at evaluating the in-vitro anti-inflammatory potential of crude extracts of Euphorbia lateriflora.
Place of Study: Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.
Methodology: The in-vitro anti-inflammatory activity of aqueous and ethanol extracts of E. lateriflora were evaluated via anti-lipoxygenase and red cell membrane stabilization assays using spectrophotometric technique.
Results: The aqueous and ethanol extracts demonstrated significant anti-lipoxygenase activity with the aqueous extracts (IC50: 0.34 mg/ml) and the ethanol extracts (IC50: 0.25 mg/ml), showing comparable activity with standard indomethacin (0.27 mg/ml). Contrary to what was observed in the anti-lipoxygenase study, the aqueous extract demonstrated a better membrane stabilization activity with IC50 (1.13 mg/ml) than the ethanol extracts (5.44 mg/ml). The standard diclofenac drug had an IC50 of 1.02 mg/kg. Both the aqueous and the ethanol extracts demonstrated significant anti-inflammatory activity.
Conclusion: Observation from this study established the considerable anti-inflammatory potential of this plant by inhibiting lipoxygenase activity and stabilization the membrane of the red blood cells.