Open AccessInfluence of Antioxidant Supplementation on High Fat Diet-Streptozotocin (HFD-STZ) Induced Type 2 Diabetes Mellitus in Albino Rats
Author(s) -
L. C. Chuku,
N. C. Chinaka
Publication year - 2020
Publication title -
european journal of medicinal plants
Language(s) - English
Resource type - Journals
ISSN - 2231-0894
DOI - 10.9734/ejmp/2020/v31i1730333
Subject(s) - antioxidant , chemistry , streptozotocin , glutathione , malondialdehyde , medicine , oxidative stress , glutathione reductase , endocrinology , glutathione peroxidase , superoxide dismutase , diabetes mellitus , vitamin e , biochemistry , enzyme
The influenceof antioxidant supplementation on high fat diet-streptozotocin (HFD-STZ) induced type 2 diabetes mellitus in Wistar albino rats was investigated. Appropriate (RDA) proportions of some antioxidant rich substances which includes; vitamins (A, B3, B6, B12, C and E), minerals (calcium, selenium, chromium, magnesium, potassium and zinc), α-lipoic acid, cinnamon powder, curcumin (Meriva®), cordyceps, resveratrol, quercetin, D-ribose-L-cysteine were pulled together in corn oil and stored at 4°C for use. Serum glutathione (GSH) and malondialdehyde (MDA) levels, as well as activities of antioxidant enzymes: superoxide dismutases (SOD), catalase (CAT), glutathione-s-transferase (GST), glutathione peroxidise (GPx) and glutathione reductase (GR) were measured using standard methods. Data analysis was done with SPSS version 20.0 and significant level was set at P≤0.05. Results of in vitro oxidative stress indices and antioxidant enzyme activity indicate that after 4 weeks of treatment, there was no significant change (p≥0.05) in serum FBS levels of treated groups compared to the normal control group, but there was a significant decrease (p≤0.05) after 8 and 12 weeks of treatment when compared to the diabetic control group. There was no significant difference (p≥0.05) in the activities of antioxidant enzymes when compared to the normal control group, while in the diabetic control there was significant increase (p≤0.05) compared to the other groups. The results after 4, 8 and 12 weeks of treatment showed a significant increase (p≤0.05) in serum GSH level of normal and treated groups compared to diabetic control group, whereas there was a significant decline (p≤0.05) in serum MDA level of treated and normal control groups when compared to diabetic control. The results therefore suggest that the supplement may possess significant (p≤0.05) free radical scavenging potentials which could be beneficial to health.