Open AccessAcute and Sub-acute Toxicity Studies of Ethanol Seed Extract of Raphia hookeri on Swiss Albino Rats
Author(s) -
G. O. Mbaka,
Steve Ogbonnia,
F O Awoyemi
Publication year - 2014
Publication title -
journal of pharmaceutical research international
Language(s) - English
Resource type - Journals
ISSN - 2456-9119
DOI - 10.9734/bjpr/2014/9330
Subject(s) - acute toxicity , creatinine , bilirubin , albumin , toxicity , cholesterol , median lethal dose , urea , lipid profile , dose dependence , body weight , medicine , high density lipoprotein , endocrinology , zoology , pharmacology , chemistry , biology , biochemistry
Aim: To evaluate the acute and sub-acute toxicities of Raphia hookeri (Rh) seed hydroethanolic extract on experimental animals. Materials and Methods: Acute toxicity study was evaluated on Swiss albino mice of both sexes. Administration of a single dose of 4000mg/kg of Rh seed extract by gavages to five mice showed no mortality, hence, its 1/20th dose was used as the highest therapeutic dose. The intra-peritoneal administration produced dose dependent mortality with median lethal dose (LD50) of approximately 323.6mg/kg body weight (bwt). In sub-acute toxicity study, Wistar rats received daily administration of the extract in the dose range of 50 to 200mg/kgbwt for 30 days. The effects on biochemical, histological and haematological parameters were evaluated. Results: The animals exhibited dose dependent body weight changes. There were some organs weight gains with the exception of the liver and testes which showed comparably lower weight compared to the control. There was a significant (p<0.05) increase in total protein, aspartate aminotransferase (AST) and albumin levels compared to the control while bilirubin and alanine aminotransferase (ALT) levels decreased appreciably at the highest extract dose. The urea level decreased while the creatinine level increased in dose dependent manner. In lipid profile study, total cholesterol, triglycerides and low density lipoprotein cholesterol (LDL-cholesterol) levels showed significant (p<0.05) decrease in value. There was significant (p<0.05) increase in high density lipoprotein cholesterol (HDL-cholesterol). Marked decrease in red blood cells, haemoglobin and haematocrit occurred. The white blood cells also decreased while neutrophil and lymphocytes increased appreciably. The extract caused marked deleterious effect on the testes leading to drastic reduction in sperm cells. Conclusion: The extract caused undesirable effect on the male reproductive organ of the animals making it unsafe for consumption by males of reproductive age.