Open AccessA Study on Keratin-Associated Protein (KAP) 3.2 Gene and Its Polymorphism in Sandyno Breed of Sheep
Author(s) -
R. Bharathesree,
R. Saravanan,
M. Jeyakumar,
Nagarajan Muthialu
Publication year - 2020
Publication title -
biotechnology journal international
Language(s) - English
Resource type - Journals
ISSN - 2456-7051
DOI - 10.9734/bji/2020/v24i430112
Subject(s) - breed , locus (genetics) , biology , genetics , allele , single strand conformation polymorphism , genotype , gene , population , polymorphism (computer science) , genomic dna , primer (cosmetics) , allele frequency , veterinary medicine , microbiology and biotechnology , exon , chemistry , medicine , environmental health , organic chemistry
The current study investigates the polymorphic patterns of keratin-associated protein (KAP) 3.2 gene in Sandyno breed of sheep. Genomic DNA was isolated from blood samples of 51 numbers of Sandyno breed. Ovine specific primer associated PCR amplification of KAP 3.2 gene revealed product at 393 bp and genotyped by PCR-SSCP (Single Strand Conformation Polymorphism) method and visualized under silver staining technique. KAP 3.2 gene locus revealed 3 genotypes, viz. AA, AB and BB with a frequency of 0.84, 0.16 and 0 in Sandyno breed with allele frequencies of A(0.92) and B(0.08). Regarding population genetic indices, the effective number of alleles (Ne) for KAP 3.2 in Sandyno breed of sheep was found to be 1.1716. The PIC values was 0.1356 and FIS values was negative (– 0.0864) in this breed. The result revealed that the selected population of Sandyno breed of sheep was in Hardy-Weinberg equilibrium without any significant deviation from the population mean and was monomorphic for KAP 3.2 gene.