Open AccessEffect of Silver Nanoparticles Synthesized from Azadirachta indica Leaves Extract on Sickle Erythrocyte Membrane Stability
Author(s) -
Mustapha Isah,
Sumayya Ahmed Ayuba
Publication year - 2022
Publication title -
asian journal of biochemistry, genetics and molecular biology
Language(s) - English
Resource type - Journals
ISSN - 2582-3698
DOI - 10.9734/ajbgmb/2022/v10i130233
Subject(s) - erythrocyte fragility , azadirachta , lysis , erythrocyte membrane , congo red , silver nanoparticle , membrane , chemistry , traditional medicine , biochemistry , nanoparticle , medicine , hemolysis , immunology , materials science , nanotechnology , adsorption , organic chemistry
Aim: To synthesize silver nanoparticles (AgNPs) from Azadirachta indica leaf extract and determine its effect on membrane stability.
Place and Duration of Study: Department of Biochemistry, Sokoto State University, Sokoto, Nigeria, between January 2021 and April 2021.
Methodology: Simple characterization using uv-vis spectroscopy was conducted and the well-known method for osmotic fragility was employed.
Results: Surface plasmon excitation for conversion of Ag+ to AgNPs was successfully achieved at 320 nm. The result revealed increase in membrane stability with 67.50 % for sickle erythrocyte (HbSS), 33.33 % for carrier erythrocyte (HbAS) and 23.33 % for normal erythrocyte (HbAA); thereby decreasing the erythrocyte lysis in the order of HbSS>HbAS>HbAA.
Conclusion: AgNPs synthesized from A. indica reduces osmotic fragility and hence increases membrane stabilizing potential particularly against sickle erythrocyte.