Open AccessIsolation and Biochemical Characterization of Cellulase Produced by Bacterial Isolates from Sugarcane Waste Soil
Author(s) -
A. U. Hassan,
R. Jafaru,
Inés Mato,
Ebipade Kereakede,
A. H. Galadanci,
A. H. Madu,
Imelda Ada Oyong,
Kingsley Onuh,
Ugochukwu Okechukwu Ozojiofor
Publication year - 2021
Publication title -
asian journal of biochemistry, genetics and molecular biology
Language(s) - English
Resource type - Journals
ISSN - 2582-3698
DOI - 10.9734/ajbgmb/2021/v9i230213
Subject(s) - cellulase , cellulose , enzyme , bacteria , biomass (ecology) , food science , chemistry , enzyme assay , reducing sugar , substrate (aquarium) , biochemistry , biology , sugar , agronomy , ecology , genetics
Cellulase is one of the most economically important enzyme, which aids in catalyzing cellulolysis, the decomposition of cellulose and other related polysaccharides. So the demand/importance of this enzyme in both domestic and commercial sectors cannot be over emphasized. In this research cellulase-producing bacteria were isolated from soil around sugarcane waste dumping area, which was identified to be P. fulorescens after numerous biochemical and microbiological analysis. The bacteria were then grown and used to ferment certain biomass, with the aim of using the organisms to produce the cellulase enzyme. The total protein/cellulase enzyme activity of the medium was ascertained. Optimization/characterization for maximum cellulase activity was done by varying the temperature, pH, enzyme concentration and substrate concentration, in which the optimum condition for cellulase production was ascertain to be at a temperature and pH of 40˚C and pH 7 respectively. SDS-PAGE electrophoresis was carried out to determine and reconfirm the presence and molecular weight of the isolated enzyme. The estimated extrapolated molecular weight of the enzyme was found to be 13.5KDa.