Open AccessMolecular Diversity Analysis of Indigenous Potato Cultivars Using RAPD Markers
Author(s) -
Farhaznin,
Md. Ashraful Haque,
Md. Hasan Sofiur Rahman,
Md. Hasan Atikur Rahman,
Md. Nazmul Hasan Mehedi
Publication year - 2020
Publication title -
asian journal of biochemistry, genetics and molecular biology
Language(s) - English
Resource type - Journals
ISSN - 2582-3698
DOI - 10.9734/ajbgmb/2020/v6i130145
Subject(s) - genetic diversity , cultivar , rapd , biology , germplasm , genetic distance , genetic divergence , ethidium bromide , genotype , genetic marker , horticulture , veterinary medicine , microbiology and biotechnology , botany , genetic variation , genetics , dna , gene , medicine , population , demography , sociology
The study was undertaken to identify genotypic diversity at molecular level of potato for varietal improvement program at the Advanced Plant Breeding Laboratory, Department of Genetics and Plant Breeding, Bongobondhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur, Bangladesh. Eight cultivars of potato viz. Lalpakri, Sindurkouta, Indurkani, Ausha, Patnai, Sadaguti, Shilbilati, Challisha were collected from Bangladesh Agricultural Research Institute (BARI), for studying genotyping divergence. Genomic DNA was extracted from young leaves of the cultivars and PCR reaction was performed. The PCR amplified DNA profile was visualized on agarose gel, staining with ethidium bromide. Three RAPD primers named OPA 01, OPA 03 and OPD 18 were used to evaluate the genetic diversity of potato varieties. All the primers were polymorphic and the primers produced the highest number of alleles. The genetic diversity value in Lalpakri with all cultivars except Sadaguti was found to have the highest genetic distance (1.0). The amount of genetic diversity within potato germplasm is quite distinct as revealed by the genetic similarity coefficients. The results indicate that, high level of genetic distance exists among the cultivars. The Primers OPA 01, OPA 03 and OPD 18 showed the highest level of genetic diversity and PIC value while the Lalpakri and Sadaguti cultivars had the highest genetic distance among other cultivars which could be used for further potato breeding program.