Open AccessChemical Composition of Essential Oil of Ageratum conyzoides with Antifungal Activity on the Lasiodiplodia theobromae Strain in the Region of Kisangani and DR Congo
Author(s) -
J. T. K. Kwembe,
O. Onautshu,
P. T. Mpiana,
Pieter Vermeir,
Geert Haesaert
Publication year - 2021
Publication title -
archives of current research international
Language(s) - English
Resource type - Journals
ISSN - 2454-7077
DOI - 10.9734/acri/2021/v21i130222
Subject(s) - ageratum conyzoides , essential oil , lasiodiplodia theobromae , potato dextrose agar , horticulture , antifungal , traditional medicine , botany , composition (language) , biology , agar , chemistry , microbiology and biotechnology , medicine , bacteria , weed , linguistics , philosophy , genetics
Aims: To determine the chemical composition of essential oil of Ageratum conyzoides with antifungal activity on the strain of Lasiodiplodia theobromae in the Kisangani region.
Location and Duration of Studies: Faculty of Sciences of University of Kisangani (Democratic Republic of Congo) and Faculty of Biosciences Engineering of University of Ghent (Belgium), between May to November 2019.
Methodology: The essential oil from the leaves of A. conyzoides was extracted by hydrodistillation. Potato dextrose agar (PDA) was used as a culture medium. In vitro evaluation of its antifungal activity was performed on PDA medium and expressed as percentage of inhibition (PI).
Results: The extracted essential oil (with a yield of 0.63%) showed a PI of 91.63% on the strain of L. theobromae after two days of incubation. It consists of at least 23 compounds, of which seven are in the majority (abundances greater than 1.5% and represent 92.05%), namely Precocene I (38.33%), Beta-caryophyllene (26.51%), Beta-sesquiphellandrene (8.63%), Beta-cubebene (7.91%), Alpha-muurolene (4.95%), 1.5.9.9-Tetramethyl-1.4.7-cycloundecatriene (3.04%), Cis-beta-farnesene (2.99%). The hydrocarbon sesquiterpenes are the most frequent compounds (58.95%).
Discussion: Compared to the A. conyzoides saponins the PI obtained from the essential oil is higher. The essential oil chemotype obtained from the leaves of A. conyzoides is of the Precocene I type, similar to the oils from the stems and flowers of the same plant in West Africa.
Conclusion: The essential oil of A. conyzoides has a very high inhibitory power on L. theobromae. It mainly contains the Precocene and the hydrocarbon sesquiterpenes. Assessment of the antifungal activity of each separate molecule should be considered.