Open AccessEFFECTS OF EXTRACTS OF HYPODAPHNIS ZENKERI AND XYLOPIA AETHIOPICA ON BLOOD LIPIDS, GLYCEMIA AND BODY WEIGHT OF TRITON WR1339 AND INSULINO RESISTANT RATS
Author(s) -
Etoundi Omgba Cunégonde Blanche,
Dieudonné Kuate,
Ngondi Judith Laure,
Oben Enyong Julius
Publication year - 2013
Publication title -
international journal of research in ayurveda and pharmacy
Language(s) - English
Resource type - Journals
eISSN - 2277-4343
pISSN - 2229-3566
DOI - 10.7897/2277-4343.04523
Subject(s) - abts , chemistry , catechin , antioxidant , phytochemical , food science , traditional medicine , hyperlipidemia , flavonols , polyphenol , dpph , diabetes mellitus , biochemistry , biology , medicine , endocrinology
Obesity is a complicated multifaceted problem generally accompanied by disturbances in carbohydrate and fat metabolism, insulin resistance as well as an increase in oxidative stress. The bark of Hypodaphnis zenkeri (HZ) and dry fruits of Xylopia aethiopica (XA) commonly used as spices in Cameroonian cuisine also have applications in traditional medicine. This study evaluates their effects on some biological parameters of acute hyperlipidemic rats and on rats fed with High Fat High Sucrose (HFHS) diet. The spices were extracted in water, water/ethanol (50/50) and ethanol. The first part of the work was the in vitro study where, the partial phytochemical composition as well as the antioxidant potential of extracts were studied, using folin Ciocalteu’s phenol method and ABTS (3-ethyl-benzothiazoline-6-sulfonic acid) assay. The best extract for each spice was selected and used for the “in vivo” study which consisted in studying their effect on weight and biochemical parameters in acute hyperlipidemic rats and insulin resistant rats. The extracts contained phenols like flavonoids. They also showed free radical scavenging properties in vitro. Total phenolic content were higher particularly for the hydroethanolic extract (HEE) of Hypodaphnis zenkeri (413.87 ± 13.19 mg Eq catechin/g of extract), but lowest for the water extract (WE) of Xylopia aethiopica (63.07 ± 1.60 mg Eq catechin/g of extract). At a concentration of 400 mg/kg, the HEE extract of Hypodaphnis zenkeri showed a hypotriglyceridemic activity on rats with hypertriglyceridemia induced by triton WR-1339 unlike the EE of Xylopia aethiopica. But these two extracts inhibited weight gain and reduced postprandial glycemia in insulin resistant rats. The bark of Hypodaphnis zenkeri, and dry fruits of Xylopia aethiopica contained bioactive ingredients; they ameliorated the glucose uptake and the evolution of weight impaired by the chronic consumption of HFHS diet in rats