Open AccessQUALITATIVE AND QUANTITATIVE ESTIMATION OF A POLYHERBAL COMPOUND DASHANG LEPA
Author(s) -
Srivastava Ashish,
Smriti Gupta,
Amit Kumar,
Yamini B. Tripathi
Publication year - 2013
Publication title -
international journal of research in ayurveda and pharmacy
Language(s) - English
Resource type - Journals
eISSN - 2277-4343
pISSN - 2229-3566
DOI - 10.7897/2277-4343.04520
Subject(s) - estimation , traditional medicine , medicine , engineering , systems engineering
In Ayurvedic system of medicine mainly polyherbal compounds are used for the treatment of various infections. Dashang lepa is one of the polyherbal compound which contain mixture of ten indigenous drugs. It is used to treat inflammatory swellings like as cellulitis by external application. As several study show qualitative and quantitative estimation of individual drug but none of the estimation involves combined study of Dashang lepa. Qualitative phytochemical, thin layer chromatography and quantitative loss on drying, ash value, pH value analysis were studied for polyherbal drug Dashang lepa. Methanolic extraction was carried out by hot percolation method through soxhlet apparatus. Phytochemical study was performed using various standard methods and TLC analysis was done with four type of solvent system. Determination of moisture content (loss on drying), measurement of ash value and pH value were also carried out as per standard guidelines of Ayurvedic Pharmacopoeia of India. The phytochemical analysis showed the presence of alkaloid, saponin, tannins, terpenoids, steroids, glycosides, phenols and flavonoids. TLC analysis shows six spots using chloroform: ethyl acetate: methanol (6: 3: 1) and toluene: chloroform: methanol (6: 3:1) two different solvent system. Loss on drying, ash value and pH were obtained 9 %, 8 % and 5.5 respectively