TLC SEPARATION, ANTIBACTERIAL AND ANTI-INFLAMMATORY ACTIVITY OF EXTRACTS DERIVED FROM ZANTHOXYLUM HUMILE ROOTS

Internationally there is an increased need to come up with new therapeutics to counteract cases of microbial resistance which is diminishing successes of current pharmaceuticals. Traditional medicines have been in use since time memorial and they offer better options as a source of new lead compounds as compared to synthetic designs and libraries. The aim of this study was to evaluate antibacterial and anti-inflammatory activity of TLC isolated phytocompounds from Zanthoxylum humile roots. Disc diffusion method was employed to determine antibacterial activity while micro-plate serial dilution method was used to evaluate the minimum inhibitory concentration of crude fractions. Anti-inflammatory activity was ascertained by the nitrite assay. The present study results reveals that Zanthoxylum humile roots consist of antibacterial and anti-inflammatory activity against both gram positive and gram negative bacteria. The antibacterial and anti-inflammatory activity can be explained in terms of alkaloids, saponins, tannins and flavonoids present in the extracts. Zanthoxylum humile roots TLC isolates revealed significantly greater antibacterial and anti-inflammatory activity as compared to standard drugs cotrimoxazole and indomethacin. Results of the present study authenticate the use of Zanthoxylum humile roots in primary health care needs. Presence of phytocompounds such as flavonoids, saponins, tannins, alkaloids, terpenoids and steroids supports its use as medication for diarrhea, hypertension, diabetes, coughs and flu and as antivenins against snake bites. The presence of various bioactive compounds makes Zanthoxylum humile a promising plant as a source of new therapeutics that may be the answer to the current microbial resistance