Open AccessMETHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF OFLOXACCIN AND SATRANIDAZOLE BY USING RP-HPLC
Author(s) -
Kalakonda Sri Nataraj,
A. Srinivasa Rao,
Nagesh Kumar Mutyala,
P. Sivalingachari,
K. Alekhya
Publication year - 2013
Publication title -
international research journal of pharmacy
Language(s) - English
Resource type - Journals
ISSN - 2230-8407
DOI - 10.7897/2230-8407.04848
Subject(s) - chromatography , high performance liquid chromatography , phosphoric acid , chemistry , acetonitrile , retention time , chromatographic separation , organic chemistry
A new simple, accurate, rapid and precise gradient High Performance Liquid Chromatographic (HPLC) method was developed and validated for simultaneous estimation of Ofloxaccin and satranidazole in combined dosage form. The method employs waters HPLC system on Hypersil BDS C18 Column (250 mm X 4.6 mm, 5 µ), with a flow rate of 1.0 ml / min with a load of 10 µl. Water + OPA (Ortho Phosphoric Acid) having pH 5.0 and acetonitrile was used as a mobile phase in the composition of 60:40 % v/v. The detection was carried out by PDA detector at 295 nm. Linearity ranges for Ofloxaccin and satranidazole were 100 – 300 g / ml for Ofloxaccin, 150 – 450 g / ml for satranidazole respectively. Retention time of Ofloxaccin and Satranidazole were found to be 2.881 minutes, 4.042 minutes respectively. Percent recovery study values of Ofloxaccin and Satranidazole were found to be 99.003 – 100.5 % and 98.6 – 100.4 % respectively. This newly developed method was successfully utilized for the quantitative estimation of Ofloxaccin and Satranidazole in combined pharmaceutical dosage forms. This method was validated as per ICH guidelines