STUDY ON THE EFFECT OF GRAPE SEED PROANTHOCYANIDINS ON ADIPOCYTOKINE RECEPTORS IN DIET INDUCED FATTY LIVER DISEASE

Fatty liver is the hepatic component of the metabolic syndrome. Compelling evidence demonstrate that cytokines produced by the adipocytes play a major role in the pathogenesis of fatty liver disease. This study investigates effect of grape seed proanthocyanidins (GSP, 100 mg / kg b.w) in comparison and in combination with metformin (MET, 50 mg / kg b.w) on the mRNA levels in liver of major receptors of adipocytokines [tumor necrosis factor (TNF)-α, interleukin (IL)-6, adiponectin and leptin] in a rat model of high fructose, fat diet (HFFD)-induced fatty liver disease. HFFD-fed rats showed increased lipid content and oxidative stress with decreased antioxidants. The mRNA levels of receptors of TNF-α, IL-6 and leptin were also higher whereas that of adiponectin was lower during fatty liver disease. Upon GSP and MET administration, these changes in the receptor expression of adipocytokines were reversed. When both MET and GSP were administered, the effects were observed to be more intense. Restoration of the levels of adipocytokine’s receptors gives a clue, in part, of the ability of GSP and MET to combat fatty liver disease