ANTIBACTERIAL EFFICACY OF MANIBADRA CHOORNAYA AND ITS INDIVIDUAL INGREDIENTS

The present study was carried out in order to find out the antibacterial activity of hot and cold extracts of Manibadra Choornaya and its ingredients; fruits of Embelia ribes, Terminalia chebula and Emblica officinalis and root of Operculina turpethum against Staphylococcus aureus (NCTC6571) and Escherichia coli (ATCC25922). Streptomycin and sterile distilled water were used as standard and control respectively. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the extracts were determined by macro broth dilution method. Phytochemical analysis was done to identify the presence of chemicals in each extracts. Quantitative and comparative evaluation of antibacterial properties showed that the hot and cold extracts of Terminalia chebula revealed significantly (P < 0.05) highest activity on both of the tested bacteria. Except Operculina turpethum, all the tested hot extracts including Manibadra Choornaya extracts exhibited better effect on Escherichia coli when compared with respective cold extracts. The same tendency was produced on Staphylococcus aureus by Terminalia chebula and the Manibadra Choornaya. Terminalia chebula hot extract showed the lowest MIC (7.8125 mg / ml) on both of the test bacteria and lowest MBC (15.625 mg / ml) on E. coli. Most of the remaining extracts had 31.25 mg / ml as their MIC and 62.5 mg / ml as MBC. The phytochemical analysis revealed the presence of tannins in all test extracts where as glycoside, saponins, phlobatannins, resins and terpenoids were found in some of the extracts. Present study prove the antibacterial activity of Manibadra Choornaya and its individual ingredients, further studies could provide more evidences for developing antimicrobial compounds from these extracts