Open AccessA COMPARATIVE ANALYSIS ON CALCIUM RELEASE FROM ENAMEL AFTER TREATMENT WITH VARIOUS REMINERALIZING AGENTS AT A PH 4.5 : AN ATOMIC ABSORBTION SPECTROMETRIC ANALYSIS
Author(s) -
Shishir Ram Shetty,
Mithra N Hegde,
P B Thimmaiah,
Smitha Sammith Shetty,
Mahesh Bekal
Publication year - 2013
Publication title -
international research journal of pharmacy
Language(s) - English
Resource type - Journals
ISSN - 2230-8407
DOI - 10.7897/2230-8407.04716
Subject(s) - remineralisation , enamel paint , calcium , chemistry , dentistry , chromatography , medicine , organic chemistry
Focus of this study was to compare the amount of calcium released from enamel treated with different remineralizing agents at pH 4.5 using atomic spectrophotometric analysis. The present study was designed to assess the calcium release from enamel when subjected to an acid challenge. Enamel samples were divided into four groups of which intact enamel served the purpose of control group and other three groups were based on the remineralizing agents used (CPP-ACP [GC Tooth Mousse], CPP-ACPF [GC Tooth Mousse Plus], 0.044 % Sodium Fluoride [Phosflur]). All the groups of enamel samples were initially demineralized and followed up with remineralisation by adopting the pH cycling model. Acetate buffer was prepared at a specific pH of 4.5. The enamel samples were subjected to acid challenge in presence of a magnetic stirrer and buffer solution were pipetted at specific time intervals for pH 4.5. The solutions collected were transferred into sterile containers and subjected to atomic spectrometry analysis