Open AccessASSESSMENT OF SPASMOLYTIC ACTIVITY OF ALCOHOLIC EXTRACT OF ACILLEA MILLEFOLIUM, RUBIA CORDIFOLIA AND SAUSSUREA LAPPA IN WISTAR ALBINO RATS
Author(s) -
Ashish Nagar,
Ashok Kumar,
Sumeet Gullaiya,
V. B. Singh,
Vishal Dubey,
B.P. Srinivasan,
Pushp Vihar
Publication year - 2013
Publication title -
international research journal of pharmacy
Language(s) - English
Resource type - Journals
ISSN - 2230-8407
DOI - 10.7897/2230-8407.04714
Subject(s) - traditional medicine , chemistry , medicine
The present study was carried out to evaluate the potential of alcoholic extract of anti-inflammatory plants viz. Achillea millefolium, Rubia cordifolia and Saussurea lappa for spasmolytic activity in rat model using radnoti organ bath. The relaxant effect of all extracts on pre-contracted rat tracheal chain by carbachol (30 µM) had been screened. Isometric contractions of isolated rat tracheas were recorded at 1.4 g resting tension and carbachol dose-response curves were performed. EC50 values (27.12, 13.13 and 7.32 µM, respectively) were identified by plotting cumulative concentration response curve and pD2 values (4.48 ± 0.99, 6.06 ± 1.03 and 7.41 ± 0.97, respectively) were calculated for individual alcoholic extract of plant. All extracts were able to relax carbachol pre-contracted tracheas significantly in a concentration dependent manner. Our results suggested potential role of Achillea millefolium, Rubia cordifolia and Saussurea lappa in asthma for further potential therapeutic and clinical uses