Open AccessEVALUATION OF ANTI-OXIDANT, CYTO-TOXIC AND ANTI-MICROBIAL PROPERTIES OF DRYNARIA QUERCIFOLIA
Author(s) -
Milon Chandra Mohanta,
Avijit Dey,
Shibley Rahman,
Rezwasreen Chowdhury
Publication year - 2013
Publication title -
international research journal of pharmacy
Language(s) - English
Resource type - Journals
ISSN - 2230-8407
DOI - 10.7897/2230-8407.04710
Subject(s) - anti oxidant , traditional medicine , chemistry , food science , toxicology , biology , medicine , biochemistry , antioxidant
Drynaria quercifolia is a plant having wide spread ethno pharmacological uses. The aim of this study was to evaluate the anti-oxidant, anti-microbial and cyto-toxic activities of the whole plant Drynaria quercifolia. The rhizome of the plant was sun dried and extracted using methanol. Later the crude methanolic extract was fractionated into four different fractions using Petroleum ether, carbon-tetrachloride, Ethyl acetate and water. The anti-oxidant activity of the different fractions was measured by the DPPH free radical scavenging activity. The ethyl acetate, carbon-tetrachloride and showed very potent anti-oxidant activity by the DPPH free radical scavenging method. The anti-microbial activity of the different fractions was measured by disc diffusion method using ciprofloxacin as a standard. In vitro antimicrobial screening of the plant showed that ethyl acetate and the carbon tetra chloride fractions showed mild anti-microbial activity. Evaluation of cyto-toxic activity was done using the brine-shrimp lethality bio-assay. The petroleum ether soluble fraction showed significant cyto-toxic potential with LC50 values of 5.59 µg / ml