Open AccessANTICANCER POTENTIAL OF METHANOLIC AND AQUEOUS EXTRACT OF LEUCAS INDICA (LINN.) AGAINST EHRLICH ASCITES CARCINOMA CELLS ON SWISS ALBINO MICE
Author(s) -
Mahananda Sarkar,
Mousumi Das,
Debmalya Mitra,
Aditya Kumar Jena,
Arnab De,
Amalesh Samanta
Publication year - 2013
Publication title -
international research journal of pharmacy
Language(s) - English
Resource type - Journals
ISSN - 2230-8407
DOI - 10.7897/2230-8407.04641
Subject(s) - ehrlich ascites carcinoma , traditional medicine , aqueous extract , leucas , pharmacology , ascites , chemistry , biology , medicine , biochemistry , in vitro , fishery
The present study was designed to evaluate the in vivo anticancer activity of methanolic and aqueous extract of Leucas indica Linn. (Aerial parts) against Ehrlich’s Ascites Carcinoma (EAC) on Swiss albino mice. The anticancer activity was done by intra peritoneal transplantation of EAC cells into mice and subsequently analysis of ascitic fluid, haematological, biochemical and histopathological parameters along with body weight and percentage of life span. The animals were treated with the extracts at the dose level of 200 and 400 mg/kg, intra peritoneally and compared to the reference drug vinblastine. Both the extracts showed significant dose dependent reduction of viable cells (cancer cells), increased non-viable cells (dead cells), decreased in body weight and increased the duration of life span. In case of hematological study the extracts showed increased hemoglobin level and RBC count but decreased WBC count. The certain important serum enzymes like glutamate oxaloacetate transaminase, glutamate pyruvate transaminase and alkaline phosphatase levels were also decreased in treated group of animals. Finally, in case of histopathological investigation of livers showed a significant protection and regeneration of EAC induced oxidative stress and cellular damage. The preliminary phytochemical screening of the extracts revealed the presence of flavonoids, total phenolic compounds, saponin and tannin. However the aqueous fraction of the Leucas indica possesses more potential anticancer property compared to methanolic fraction. The anticancer property of this plant was considered due to the presence of flavonoids and total phenolic compounds