Open AccessSCREENING OF ENDOPHYTIC FUNGI FROM CASSIA SIAMEA LAMK LEAVES AS α-GLUCOSIDASE INHIBITOR
Author(s) -
Abdul Mun’im,
Mohammed Ramadhan,
Atiek Soemiati
Publication year - 2013
Publication title -
international research journal of pharmacy
Language(s) - English
Resource type - Journals
ISSN - 2230-8407
DOI - 10.7897/2230-8407.04526
Subject(s) - cassia , plant use of endophytic fungi in defense , traditional medicine , botany , biology , chemistry , medicine , alternative medicine , pathology , traditional chinese medicine
Alpha glucosidase inhibitor is one of therapeutic approaches for diabetes mellitus which is known for its safety compare to other oral anti diabetic drugs. Therefore searching of α-glucosidase inhibitor from natural compounds was recently done by many researchers to find the new active compounds. Endophytic fungi have great potential as a source of α-glucosidase inhibitory compounds. This research aims to isolate the endophytic fungi from Cassia siamea Lamk. leaves and then to evaluate their α-glucosidase inhibitory activity. We successfully isolated five endophytic fungi colonies, and then each isolate was fermented and extracted with ethyl acetate and methanol. Each extract was assayed for its α-glucosidase inhibitory activity using spectrophotometry method. Nine extracts showed better α-glucosidase inhibitory than acarbose with the smallest IC50 value was 28.40 ppm